When un-germinated seeds were moved from 5–10 ☌ to 15 ☌ for a further 28 days, germination increased from 0 to >80 % in significantly less time indicating that cold stratification may play a key role in the germination process during winter and early spring in A. Embryo length significantly increased at 10 ☌, and only slightly increased at 5 ☌, while growth did not occur at 25 ☌. After-ripened seeds germinated to ≥80 % at 15–20 ☌ while no additional benefit of germination was observed in the presence of the KAR 1 or glyceronitrile.
flavidus had MPD and the physiological dormancy (PD) component could be broken by either glyceronitrile or dry after-ripening. Results demonstrated that fresh seeds of A.
Embryo growth dynamics were tested at 5, 10, 15 and 25 ☌. After incubation at 5, 10 and 25 ☌ for 28 days, seeds were transferred to 15 ☌ for another 28 days. flavidus, after-ripened for 28 months at 15 ☌/15 % relative humidity, were incubated on water agar, water agar containing 1 μM karrikinolide (KAR 1) or 50 μM glyceronitrile at 5, 10, 15, 20, 25, 20/10 and 25/15 ☌ for 28 days. Anigozanthos flavidus (Haemodoraceae) produces seeds with morphophysiological dormancy (MPD) that are known to germinate in response to smoke, but embryo growth dynamics and germination traits in response to temperatures and after-ripening have not been well characterized. Seed dormancy status regulates the response of seeds to environmental cues that can trigger germination.
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